Poster Presentation 6th Australian Health and Medical Research Congress 2012

DNA copy number variations of a novel gene JS-1 in colorectal adenocarcinomas (#306)

Vinod Gopalan 1 , Suja Pillai 2 , Johnny Chuek-On Tang 1 , Robert A Smith 1 , Alfred K-Y Lam 1
  1. School of Medicine and Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia
  2. Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, Hong Kong SAR

JS-1 is a novel gene located at 5p15.2 and originally detected in primary oesophageal cancer.  Our previous study reported frequent overexpression of JS-1 in oesophageal cancers and confirmed its tumour transforming capacity in normal cells, indicating a potential role in the molecular carcinogenesis of human cancers. The aim of this study was to determine the gene copy number JS-1 in a small cohort of patients with colorectal tumours. We evaluated the DNA copy number of JS-1 in 40 colorectal tissues (30 adenocarcinomas, 10 non-neoplastic tissues) using real-time polymerase chain reaction. JS-1 DNA was detected in all selected colorectal cancer and control tissues. Copy number for JS-1 was significantly higher in colorectal cancers compared to the non-cancer control tissues (p=0.003). Among the cancers, 97% (n=27/30) showed high JS-1 copies at the DNA level when compared to the control tissues.  This is the first study showing significant correlation of JS-1 copy number with malignancy in colorectal tissues. High gain of JS-1 copies in colorectal cancers confirms our earlier finding of the oncogenic properties of JS-1 in oesophageal cancers. To conclude, these results suggest that JS-1 is involved in the pathogenesis of colorectal cancers and future research needs to be undertaken to comprehend the role of this novel gene in molecular carcinogenesis.